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Simulation and evaluation of 3D traction force microscopy.
Journal article

Simulation and evaluation of 3D traction force microscopy.

  • Holenstein CN a Department of Orthopedics , Balgrist University Hospital , Zurich , Switzerland.
  • Lendi CR a Department of Orthopedics , Balgrist University Hospital , Zurich , Switzerland.
  • Wili N a Department of Orthopedics , Balgrist University Hospital , Zurich , Switzerland.
  • Snedeker JG a Department of Orthopedics , Balgrist University Hospital , Zurich , Switzerland.
  • 2019-04-10
Published in:
  • Computer methods in biomechanics and biomedical engineering. - 2019
Traction force microscopy
digital volume correlation
evaluation
simulation
Computer Simulation
Finite Element Analysis
Imaging, Three-Dimensional
Microscopy, Atomic Force
Traction
English Measuring cell-generated forces by Traction Force Microscopy (TFM) has become a standard tool in cell mechanobiology. Although widely used in two dimensional (2D) experiments, only a few methods exist to measure traction in three-dimensional (3D) cell culture, since 3D volumetric high-resolution microscopy and more demanding computational approaches are required. Although it is commonly known that the selected experimental and computational setup highly influence the quality and accuracy of the results, no existing methods can adequately assess the errors involved in this process. We present a fully integrated simulation and evaluation platform that allows one to simulate TFM images and quantify errors of an applied approach for traction stress reconstruction, in order to improve experiments that attempt to measure mechanical interaction in cellular systems. In this context, we show that a careful parameter selection can decrease the reconstructed traction error by up to 40%.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/67354
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