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A red-fluorescent substrate microarray for lipase fingerprinting.
Journal article

A red-fluorescent substrate microarray for lipase fingerprinting.

  • Grognux J Department of Chemistry & Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland.
  • Reymond JL
  • 2007-01-12
Published in:
  • Molecular bioSystems. - 2006
Cluster Analysis
Fluorescent Dyes
Lipase
Molecular Structure
Peptide Mapping
Protein Array Analysis
Substrate Specificity
English A lipase substrate microarray was obtained by printing aliphatic C2-C12 monoesters of (5R)- and (5S)-3-(5,6-dihydroxyhexyloxy)benzaldehyde by reductive alkylation on amine-functionalized glass slides coated with bovine serum albumin and a short PEG linker. The microarray features 12 substrates and their 66 possible binary mixtures spotted in a 9 x 36 spot array. Lipase reactions are detected by chemoselective NaIO(4)-oxidation of the 1,2-diol hydrolysis product to form an aldehyde, which is then tagged with the red-fluorescent dye rhodamine B sulfohydrazide . Specific fingerprints are produced by active enzymes. These experiments provide the first example of lipase fingerprinting using microarrays.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/6768
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