The beta-isoform of neuronal nitric oxide synthase (nNOS) lacking the PDZ domain is localized at the sarcolemma.
- Baum O Department of Anatomy, University of Bern, Bern, Switzerland. oliver.baum@ana.unibe.ch
- Schläppi S
- Huber-Abel FA
- Weichert A
- Hoppeler H
- Zakrzewicz A
- 2011-09-28
Published in:
- FEBS letters. - 2011
Animals
Immunohistochemistry
Isoenzymes
Mice
Mice, Knockout
Muscle Proteins
Muscle, Skeletal
Nitric Oxide Synthase Type I
Protein Structure, Tertiary
Rats
Sarcolemma
English
In skeletal muscles, the expression of neuronal NO synthase (nNOS) isoforms is uncharacterized at the protein level. We therefore conducted epitope mapping with anti-peptide-antibodies. Antibodies specific for the nNOS N-terminus recognized the 160-kDa alpha-isoform. In contrast, antibodies against the middle portion or the C-terminus of nNOS bound additionally to the truncated 140-kDa beta-isoform which lacks the PDZ-domain present in the alpha-isoform. All nNOS immunohistochemical reactivity was confined to the sarcolemma. Consistently, immunoblotting disclosed both nNOS-isoforms to be co-enriched in the membrane-associated fractions. The beta-isoform was co-immunoprecipitated with alpha-isoform antibodies in muscle extracts indicating an association of both nNOS-isoforms to direct the beta-variant to the sarcolemma.
- Language
-
- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1016/j.febslet.2011.09.016
- PMID 21945318
- Persistent URL
- https://sonar.ch/global/documents/6794
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