The use of RNA probes for the analysis of gene expression.
- Belin D Department of Pathology, University of Geneva Medical School, CMU, Switzerland. dominique.belin@medecine.unigo.ch
- 1997-04-01
Published in:
- Molecular biotechnology. - 1997
Animals
Blotting, Northern
Gene Expression
Nucleic Acid Hybridization
Plasmids
Polymerase Chain Reaction
RNA Probes
Ribonucleases
Templates, Genetic
English
The monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unlimited quantity. In this protocol, we describe the preparation of plasmid and PCR-derived templates. A basic transcription protocol is provided with several optional modifications. The use of RNA probes in Northern blot hybridization and in RNase protection assays is described. The relative advantages and pitfalls of these two methods to quantitatively detect mRNA targets are discussed.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1007/BF02761751
- PMID 9219230
- Persistent URL
- https://sonar.ch/global/documents/70241
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