Cell-permeant and photocleavable chemical inducer of dimerization.
- Zimmermann M University of Basel, Department of Biomedicine, Mattenstrasse 28, Basel (Switzerland).
- Cal R
- Janett E
- Hoffmann V
- Bochet CG
- Constable E
- Beaufils F
- Wymann MP
- 2014-03-29
Published in:
- Angewandte Chemie (International ed. in English). - 2014
chemical inducers of dimerization
fusion proteins
photocleavable linkers
photolysis
protein-protein interactions
Cell Membrane
Cell Membrane Permeability
Cytosol
Endocytosis
Golgi Apparatus
Green Fluorescent Proteins
Guanine
HeLa Cells
Humans
Kinetics
Light
Lysosomes
Mitochondria
Photosensitizing Agents
Protein Multimerization
Protein Transport
Recombinant Fusion Proteins
English
Chemical inducers of dimerization (CIDs) have been developed to orchestrate protein dimerization and translocation. Here we present a novel photocleavable HaloTag- and SNAP-tag-reactive CID (MeNV-HaXS) with excellent selectivity and intracellular reactivity. Excitation at 360 nm cleaves the methyl-6-nitroveratryl core of MeNV-HaXS. MeNV-HaXS covalently links HaloTag- and SNAP-tag fusion proteins, and enables targeting of selected membranes and intracellular organelles. MeNV-HaXS-mediated translocation has been validated for plasma membrane, late endosomes, lysosomes, Golgi, mitochondria, and the actin cytoskeleton. Photocleavage of MeNV-HaXS liberates target proteins and provides access to optical manipulation of protein relocation with high spatiotemporal and subcellular precision. MeNV-HaXS supports kinetic studies of protein dynamics and the manipulation of subcellular enzyme activities, which is exemplified for Golgi-targeted cargo and the assessment of nuclear import kinetics.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1002/anie.201310969
- PMID 24677313
- Persistent URL
- https://sonar.ch/global/documents/72915
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