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Single-cell resolution in high-resolution synchrotron X-ray CT imaging with gold nanoparticles.

  • Schültke E Stereotactic and Functional Neurosurgery, Freiburg University Medical Center, Breisacher Strasse 64, Freiburg 79106, Germany.
  • Menk R Sincrotrone Trieste SCpA, Science Park, Trieste, Italy.
  • Pinzer B Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.
  • Astolfo A Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.
  • Stampanoni M Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.
  • Arfelli F Dipartimento di Fisica, Università di Trieste and Istituto Nazionale di Fisica Nucleare Sezione di Trieste, Trieste, Italy.
  • Harsan LA Medical Physics, Radiology Department, Freiburg University Medical Center, Freiburg, Germany.
  • Nikkhah G Neurochirurgische Klinik, Universitätsklinikum Erlangen, Schwabachanlage 6, 91054 Erlangen, Germany.
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  • 2013-12-25
Published in:
  • Journal of synchrotron radiation. - 2014
X-ray CT
animal model
brain tumour
gold nanoparticles
high-resolution imaging
Animals
Brain
Brain Neoplasms
Glioma
Gold
Magnetic Resonance Imaging
Metal Nanoparticles
Rats
Rats, Wistar
Single-Cell Analysis
Synchrotrons
Tomography, X-Ray Computed
English Gold nanoparticles are excellent intracellular markers in X-ray imaging. Having shown previously the suitability of gold nanoparticles to detect small groups of cells with the synchrotron-based computed tomography (CT) technique both ex vivo and in vivo, it is now demonstrated that even single-cell resolution can be obtained in the brain at least ex vivo. Working in a small animal model of malignant brain tumour, the image quality obtained with different imaging modalities was compared. To generate the brain tumour, 1 × 10(5) C6 glioma cells were loaded with gold nanoparticles and implanted in the right cerebral hemisphere of an adult rat. Raw data were acquired with absorption X-ray CT followed by a local tomography technique based on synchrotron X-ray absorption yielding single-cell resolution. The reconstructed synchrotron X-ray images were compared with images obtained by small animal magnetic resonance imaging. The presence of gold nanoparticles in the tumour tissue was verified in histological sections.
Language
  • English
Open access status
green
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https://sonar.ch/global/documents/765
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