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BUSCO: Assessing Genome Assembly and Annotation Completeness.
Journal article

BUSCO: Assessing Genome Assembly and Annotation Completeness.

  • Seppey M Department of Genetic Medicine and Development, Swiss Institute of Bioinformatics, University of Geneva Medical School, Geneva, Switzerland.
  • Manni M Department of Genetic Medicine and Development, Swiss Institute of Bioinformatics, University of Geneva Medical School, Geneva, Switzerland.
  • Zdobnov EM Department of Genetic Medicine and Development, Swiss Institute of Bioinformatics, University of Geneva Medical School, Geneva, Switzerland. evgeny.zdobnov@unige.ch.
  • 2019-04-26
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2019
BUSCO
Gene content
Genome completeness
Orthologs
Phylogenomics
Quality assessment
Databases, Genetic
Gene Dosage
Genome
Genomics
Internet
Markov Chains
Molecular Sequence Annotation
Software
Transcriptome
English Genomics drives the current progress in molecular biology, generating unprecedented volumes of data. The scientific value of these sequences depends on the ability to evaluate their completeness using a biologically meaningful approach. Here, we describe the use of the BUSCO tool suite to assess the completeness of genomes, gene sets, and transcriptomes, using their gene content as a complementary method to common technical metrics. The chapter introduces the concept of universal single-copy genes, which underlies the BUSCO methodology, covers the basic requirements to set up the tool, and provides guidelines to properly design the analyses, run the assessments, and interpret and utilize the results.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/76753
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