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Journal article

Characterization of the Bradyrhizobium japonicum ftsH gene and its product.

  • Narberhaus F Institut für Mikrobiologie, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland. fnarber@micro.biol.ethz.ch
  • Urech C
  • Hennecke H
  • 1999-11-26
Published in:
  • Journal of bacteriology. - 1999
ATP-Dependent Proteases
Amino Acid Sequence
Bacterial Proteins
Bradyrhizobium
Cloning, Molecular
Escherichia coli
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Heat-Shock Response
Membrane Proteins
Molecular Sequence Data
Mutation
Nitrogen
Sequence Homology, Amino Acid
Time Factors
English The Bradyrhizobium japonicum ftsH gene was cloned by using a set of widely applicable degenerated oligonucleotides. Western blot experiments indicated that the FtsH protein was produced under standard growth conditions and that it was not heat inducible. Attempts to delete the ftsH gene in B. japonicum failed, suggesting a pivotal cellular function of this gene. The expression of B. japonicum ftsH in an ftsH-negative Escherichia coli strain significantly enhanced the fitness of this mutant and reduced the steady-state level of sigma(32).
Language
  • English
Open access status
bronze
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Persistent URL
https://sonar.ch/global/documents/77870
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