CAVIN-3 regulates circadian period length and PER:CRY protein abundance and interactions.
- Schneider K Department of Molecular Biology, Sciences III, University of Geneva, Geneva 1211, Switzerland.
- Köcher T
- Andersin T
- Kurzchalia T
- Schibler U
- Gatfield D
- 2012-10-20
Published in:
- EMBO reports. - 2012
Animals
Caveolae
Circadian Clocks
Cryptochromes
Gene Expression Regulation
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Mice
NIH 3T3 Cells
Period Circadian Proteins
Protein Binding
Protein Kinase C-delta
Protein Transport
RNA-Binding Proteins
English
In mammals, transcriptional autorepression by Period (PER) and Cryptochrome (CRY) protein complexes is essential for the generation of circadian rhythms. We have identified CAVIN-3 as a new, cytoplasmic PER2-interacting protein influencing circadian clock properties. Thus, CAVIN-3 loss- and gain-of-function shortened and lengthened, respectively, the circadian period in fibroblasts and affected PER:CRY protein abundance and interaction. While depletion of protein kinase Cδ (PKCδ), a known partner of CAVIN-3, had little effect on circadian gene expression, CAVIN-3 required the PKCδ-binding site to exert its effect on period length. This suggests the involvement of yet uncharacterized protein kinases. Finally, CAVIN-3 activity in circadian gene expression was independent of caveolae.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1038/embor.2012.158
- PMID 23079727
- Persistent URL
- https://sonar.ch/global/documents/77961
Statistics
Document views: 19
File downloads:
- Full-text: 0