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Transferred cross-relaxation and cross-correlation in NMR: effects of intermediate exchange on the determination of the conformation of bound ligands.
Journal article

Transferred cross-relaxation and cross-correlation in NMR: effects of intermediate exchange on the determination of the conformation of bound ligands.

  • 2003-08-14
Published in:
  • Journal of magnetic resonance (San Diego, Calif. : 1997). - 2003
Algorithms
Binding Sites
Computer Simulation
Concanavalin A
Energy Transfer
Ligands
Macromolecular Substances
Mannose
Models, Chemical
Nuclear Magnetic Resonance, Biomolecular
Protein Binding
Protein Conformation
Proteins
Signal Processing, Computer-Assisted
Statistics as Topic
English Exchange transferred effects in solution-state NMR experiments allow one to determine the conformation of ligands that are weakly bound to macromolecules. Exchange-transferred nuclear Overhauser effect spectroscopy ('TR-NOESY') provides information about internuclear distances in a ligand in the bound state. Recently the possibility of obtaining dihedral angle information from a ligand in the bound state by exchange-transferred cross-correlation spectroscopy ('TR-CCSY') has been reported. In both cases the analysis of the signal amplitudes is usually based on the assumption that rapid exchange occurs between the free and bound forms of the ligand. In this paper we show that the fast exchange condition is not easily attained for observing exchange-transferred cross-correlation effects even in systems where exchange-transferred NOE can be observed. Extensive simulations based on analytical expressions for signal intensities corresponding to fast, intermediate, and slow chemical exchange have been carried out on a test system to determine the exchange regimes in which the fast exchange condition can be fulfilled for successfully implementing TR-NOESY and TR-CCSY.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/77970
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