Analytical techniques for multiplex analysis of protein biomarkers.
- Van Gool A Translational Metabolic Laboratory, Department of Laboratory Medicine, Radboud Institute of Molecular Life Sciences, Radboud University Medical Center , Nijmegen, The Netherlands.
- Corrales F Functional Proteomics Laboratory, Centro Nacional De Biotecnología , Madrid, Spain.
- Čolović M Department of Physical Chemistry, "Vinča" Institute of Nuclear Sciences, University of Belgrade , Belgrade, Serbia.
- Krstić D Institute of Medical Chemistry, Faculty of Medicine, University of Belgrade , Belgrade, Serbia.
- Oliver-Martos B Neuroimmunology and Neuroinflammation Group. Instituto De Investigación Biomédica De Málaga-IBIMA. UGC Neurociencias, Hospital Regional Universitario De Málaga , Malaga, Spain.
- Martínez-Cáceres E Immunology Division, LCMN, Germans Trias I Pujol University Hospital and Research Institute, Campus Can Ruti, Badalona, and Department of Cellular Biology, Physiology and Immunology, Universitat Autònoma De Barcelona , Cerdanyola Del Vallès, Spain.
- Jakasa I Laboratory for Analytical Chemistry, Department of Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb , Zagreb, Croatia.
- Gajski G Mutagenesis Unit, Institute for Medical Research and Occupational Health , Zagreb, Croatia.
- Brun V Université Grenoble Alpes, CEA, Inserm, IRIG, BGE , Grenoble, France.
- Kyriacou K Department of Electron Microscopy/Molecular Biology, The Cyprus School of Molecular Medicine/The Cyprus Institute of Neurology and Genetics , Nicosia, Cyprus.
- Burzynska-Pedziwiatr I Medical Faculty, Department of Biomedical Sciences, Chair of Medical Biology & Department of Structural Biology, Medical University of Lodz , Łódź, Poland.
- Wozniak LA Medical Faculty, Department of Biomedical Sciences, Chair of Medical Biology & Department of Structural Biology, Medical University of Lodz , Łódź, Poland.
- Nierkens S Center for Translational Immunology, University Medical Center Utrecht & Princess Máxima Center for Pediatric Oncology , Utrecht, The Netherlands.
- Pascual García C Materials Research and Technology Department, Luxembourg Institute of Science and Technology (LIST) , Belvaux, Luxembourg.
- Katrlik J Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences , Bratislava, Slovakia.
- Bojic-Trbojevic Z Laboratory for Biology of Reproduction, Institute for the Application of Nuclear Energy - INEP, University of Belgrade , Belgrade, Serbia.
- Vacek J Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University , Olomouc, Czech Republic.
- Llorente A Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital , Oslo, Norway.
- Antohe F Proteomics Department, Institute of Cellular Biology and Pathology "N. Simionescu" of the Romanian Academy , Bucharest, Romania.
- Suica V Proteomics Department, Institute of Cellular Biology and Pathology "N. Simionescu" of the Romanian Academy , Bucharest, Romania.
- Suarez G Center for Primary Care and Public Health (Unisanté), University of Lausanne , Lausanne, Switzerland.
- t'Kindt R Research Institute for Chromatography (RIC) , Kortrijk, Belgium.
- Martin P Department of Medical Oncology, Midland Regional Hospital Tullamore/St. James's Hospital , Dublin, Ireland.
- Penque D Human Genetics Department, Instituto Nacional De Saúde Dr Ricardo Jorge, Lisboa, Portugal and Centre for Toxicogenomics and Human Health, Universidade Nova De Lisboa , Lisbon,Portugal.
- Martins IL Human Genetics Department, Instituto Nacional De Saúde Dr Ricardo Jorge, Lisboa, Portugal and Centre for Toxicogenomics and Human Health, Universidade Nova De Lisboa , Lisbon,Portugal.
- Bodoki E Analytical Chemistry Department, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy , Cluj-Napoca, Romania.
- Iacob BC Analytical Chemistry Department, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy , Cluj-Napoca, Romania.
- Aydindogan E Department of Chemistry, Graduate School of Sciences and Engineering, Koç University , Istanbul, Turkey.
- Timur S Institute of Natural Sciences, Department of Biochemistry, Ege University , Izmir, Turkey.
- Allinson J Immunologix Laboratories , Tampa, FL, USA.
- Sutton C Institute of Cancer Therapeutics, University of Bradford , Bradford, UK.
- Luider T Department of Neurology, Erasmus MC , Rotterdam, The Netherlands.
- Wittfooth S Medisiina D 6 A-B, University of Turku , Turku, Finland.
- Sammar M Ephraim Katzir Department of Biotechnology Engineering, ORT Braude College , Karmiel, Israel.
- 2020-05-20
Published in:
- Expert review of proteomics. - 2020
English
INTRODUCTION
The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research.
AREAS COVERED
We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses.
EXPERT COMMENTARY
The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.
The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research.
AREAS COVERED
We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses.
EXPERT COMMENTARY
The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.
- Language
-
- English
- Open access status
- hybrid
- Identifiers
-
- DOI 10.1080/14789450.2020.1763174
- PMID 32427033
- Persistent URL
- https://sonar.ch/global/documents/77980
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