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Sample Enrichment for Bioanalytical Assessment of Disinfected Drinking Water: Concentrating the Polar, the Volatiles, and the Unknowns.
Journal article

Sample Enrichment for Bioanalytical Assessment of Disinfected Drinking Water: Concentrating the Polar, the Volatiles, and the Unknowns.

  • Stalter D National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.
  • Peters LI National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.
  • O'Malley E National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.
  • Tang JY National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.
  • Revalor M Advanced Water Management Centre (AWMC), The University of Queensland , Brisbane, Queensland 4072, Australia.
  • Farré MJ Advanced Water Management Centre (AWMC), The University of Queensland , Brisbane, Queensland 4072, Australia.
  • Watson K National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.
  • von Gunten U Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Duebendorf, Switzerland.
  • Escher BI National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.
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  • 2016-05-07
Published in:
  • Environmental science & technology. - 2016
Animals
Disinfectants
Disinfection
Drinking Water
Humans
Trihalomethanes
Water Pollutants, Chemical
Water Purification
English Enrichment methods used in sample preparation for the bioanalytical assessment of disinfected drinking water result in the loss of volatile and hydrophilic disinfection byproducts (DBPs) and hence likely tend to underestimate biological effects. We developed and evaluated methods that are compatible with bioassays, for extracting nonvolatile and volatile DBPs from chlorinated and chloraminated drinking water to minimize the loss of analytes. For nonvolatile DBPs, solid-phase extraction (SPE) with TELOS ENV as solid phase performed superior compared to ten other sorbents. SPE yielded >70% recovery of nonpurgeable adsorbable organic halogens (AOX). For volatile DBPs, cryogenic vacuum distillation performed unsatisfactorily. Purge and cold-trap with crushed ice serving as condensation nuclei achieved recoveries of 50-100% for trihalomethanes and haloacetonitriles and approximately 60-90% for purged AOX from tap water. We compared the purgeable versus the nonpurgeable fraction by combining purge-and-trap extraction with SPE. The purgeable DBP fraction enriched with the purge-and-trap method exerted a lower oxidative stress response in mammalian cells than the nonpurgeable DBPs enriched with SPE after purging, while contributions of both fractions to bacterial cytotoxicity was more variable. 37 quantified DBPs explained almost the entire AOX in the purge-and-trap extracts, but <16% in the SPE extracts demonstrating that the nonpurgeable fraction is dominated by unknown DBPs.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/80271
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