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Relevance of bioreactors and whole tissue cultures for the translation of new therapies to humans.

  • Peroglio M AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland.
  • Gaspar D Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), National University of Ireland Galway (NUI Galway), Galway, Ireland.
  • Zeugolis DI Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), National University of Ireland Galway (NUI Galway), Galway, Ireland.
  • Alini M AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland.
  • 2017-07-19
Published in:
  • Journal of orthopaedic research : official publication of the Orthopaedic Research Society. - 2018
bioreactor
ex vivo culture
three-dimensional cultures
two-dimensional cultures
whole organ culture
Animal Testing Alternatives
Animals
Bioreactors
Cartilage
Humans
Intervertebral Disc
Ligaments
Tendons
Tissue Culture Techniques
Tissue Engineering
English The purpose of this review is to provide a brief overview of bioreactor-based culture systems as alternatives to conventional two- and three-dimensional counterparts. The role, challenges, and future aspirations of bioreactors in the musculoskeletal field (e.g., cartilage, intervertebral disc, tendon, and bone) are discussed. Bioreactors, by recapitulating physiological processes, can be used effectively as part of the initial in vitro screening, reducing that way the number of animal required for preclinical assessment, complying with the 3R principles and, in most cases, allowing working with human tissues. The clinical significance of bioreactors is that, by providing more physiologically relevant conditions to customarily used two- and three-dimensional cultures, they hold the potential to provide a testing platform that is more predictable of a whole tissue response, thereby facilitating the screening of treatments before the initiation of clinical trials. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:10-21, 2018.
Language
  • English
Open access status
bronze
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https://sonar.ch/global/documents/8121
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