Journal article
Low osmolality and shear stress during liposuction impair cell viability in autologous fat grafting.
- Ismail T Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel, Basel, BS, Switzerland; Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel, BS, Switzerland. Electronic address: Tarek.Ismail@usb.ch.
- Bürgin J Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel, Basel, BS, Switzerland; Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel, BS, Switzerland.
- Todorov A Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel, BS, Switzerland.
- Osinga R Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel, Basel, BS, Switzerland.
- Menzi N Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel, BS, Switzerland.
- Largo RD Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel, Basel, BS, Switzerland; Department of Plastic Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
- Haug M Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel, BS, Switzerland.
- Martin I Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel, BS, Switzerland.
- Scherberich A Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel, BS, Switzerland.
- Schaefer DJ Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel, Basel, BS, Switzerland.
- 2017-03-22
Published in:
- Journal of plastic, reconstructive & aesthetic surgery : JPRAS. - 2017
Adipocytes
Autologous fat transfer
Liposuction
Osmolality
Stromal vascular fraction
Tumescent
Adipocytes
Adipose Tissue
Analysis of Variance
Cell Differentiation
Cell Survival
Cells, Cultured
Female
Humans
Hydrogen-Ion Concentration
Hypertonic Solutions
Hypotonic Solutions
Isotonic Solutions
Lipectomy
Middle Aged
Osmolar Concentration
Stress, Mechanical
Stress, Physiological
Stromal Cells
Tissue and Organ Harvesting
Transplantation, Autologous
English
BACKGROUND
Liposuction and subsequent autologous fat grafting have become essential techniques for fat augmentation in plastic surgery. However, standard harvesting techniques that ensure the survival of adipocytes and stromal vascular fraction (SVF) cells and thus preserve the transplanted fat volume are lacking. In particular, the effect of different parameters of the tumescent solution has not been studied in this context. We hypothesized that the osmolality of the tumescent solution could have a significant effect on the survival of adipocytes and SVF cells.
METHODS
We developed two distinct in vitro models based on freshly harvested excision fat from patients undergoing surgical treatment. First, we investigated the effect of osmolality by incubating excision fat in different tumescent solutions and analyzed the total cell survival and the differentiation potential of SVF cells. Vital whole-mount staining, isolation yield of SVF cells, clonogenicity, and osteogenic and adipogenic differentiation capacities were analyzed. Second, we addressed the additional effect of mechanical stress by simulating a liposuction on pieces of excision fat after incubation with the tumescent solutions.
RESULTS
Osmolality of the tumescent solution by itself did not have a significant effect on adipocyte and SVF viability or SVF differentiation. However, when osmolality was combined with liposuction, a significant trend toward lower viability and more lipid droplets with lower osmolality was observed. Especially, SVF viability was significantly lower after liposuction with a hypotonic (150 mOsm/kg) solution.
CONCLUSION
This study demonstrates the considerable effect of osmolality during liposuction and may lead to the development of "cell-protective" tumescent solutions.
Liposuction and subsequent autologous fat grafting have become essential techniques for fat augmentation in plastic surgery. However, standard harvesting techniques that ensure the survival of adipocytes and stromal vascular fraction (SVF) cells and thus preserve the transplanted fat volume are lacking. In particular, the effect of different parameters of the tumescent solution has not been studied in this context. We hypothesized that the osmolality of the tumescent solution could have a significant effect on the survival of adipocytes and SVF cells.
METHODS
We developed two distinct in vitro models based on freshly harvested excision fat from patients undergoing surgical treatment. First, we investigated the effect of osmolality by incubating excision fat in different tumescent solutions and analyzed the total cell survival and the differentiation potential of SVF cells. Vital whole-mount staining, isolation yield of SVF cells, clonogenicity, and osteogenic and adipogenic differentiation capacities were analyzed. Second, we addressed the additional effect of mechanical stress by simulating a liposuction on pieces of excision fat after incubation with the tumescent solutions.
RESULTS
Osmolality of the tumescent solution by itself did not have a significant effect on adipocyte and SVF viability or SVF differentiation. However, when osmolality was combined with liposuction, a significant trend toward lower viability and more lipid droplets with lower osmolality was observed. Especially, SVF viability was significantly lower after liposuction with a hypotonic (150 mOsm/kg) solution.
CONCLUSION
This study demonstrates the considerable effect of osmolality during liposuction and may lead to the development of "cell-protective" tumescent solutions.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.bjps.2017.01.023
- PMID 28320625
- Persistent URL
- https://sonar.ch/global/documents/8820
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