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Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR.
Journal article

Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR.

  • 2006-02-01
Published in:
  • FEMS microbiology letters. - 2006
Anti-Bacterial Agents
Bacterial Proteins
Drug Resistance, Bacterial
Gene Expression Regulation, Bacterial
Humans
Porins
Pseudomonas aeruginosa
Reverse Transcriptase Polymerase Chain Reaction
beta-Lactamases
English In Pseudomonas aeruginosa many of the clinically relevant resistance mechanisms result from changes in gene expression as exemplified by the Mex drug efflux pumps, the AmpC beta-lactamase and the carbapenem-specific porin OprD. We used quantitative real-time-PCR to analyze the expression of these genes in susceptible and antibiotic-resistant laboratory and clinical strains. In nalB mutants, which overexpress OprM, we observed a four- to eightfold increase in the expression of mexA, mexB, and oprM genes. MexX and mexY genes were induced eight to 12 times in the presence of 2 mg L(-1) tetracycline. The mexC/oprJ and mexE/oprN gene expression levels were increased 30- to 250-fold and 100- to 760-fold in nfxB and nfxC mutants, respectively. We further found that in defined laboratory strains expression levels of ampC and oprD genes paralleled beta-lactamase activity and OprD protein levels, respectively. Our data support the use of quantitative real-time-PCR chain reaction for the analysis of the antimicrobial resistance gene expression in P. aeruginosa.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/89222
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