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The Rip1Tag2 Transgenic Mouse Model.
Journal article

The Rip1Tag2 Transgenic Mouse Model.

  • Bill R Department of Biomedicine, University of Basel, Mattenstrasse 28, 4058, Basel, Switzerland.
  • Christofori G Department of Biomedicine, University of Basel, Mattenstrasse 28, 4058, Basel, Switzerland. gerhard.christofori@unibas.ch.
  • 2016-11-19
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2016
Anti-angiogenic therapy
Endothelial cells
Immunofluorescence
Insulinoma
PNET
Rip1Tag2
Tumor angiogenesis
Animals
Antigens, Viral, Tumor
Apoptosis
Carcinoma, Neuroendocrine
Cell Proliferation
Insulin
Insulinoma
Mice
Mice, Transgenic
Neoplasms, Experimental
Neovascularization, Pathologic
Pancreatic Neoplasms
Promoter Regions, Genetic
English The Rip1Tag2 transgenic mouse model of β-cell carcinogenesis has been instrumental in studying various aspects of tumor angiogenesis and in investigating the response to anti-angiogenic therapeutics. Thereby, the in-depth assessment of blood and lymphatic vessel phenotypes and functionality represents key experimental analyses. In this chapter, we describe basic protocols to assess tumor blood vessel morphology (pericyte coverage), functionality (perfusion, leakiness, and hypoxia), lymphatic tumor coverage, and tumor cell proliferation and apoptosis based on immunofluorescence microscopy analysis.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/90678
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