Combining Time-of-Flight Secondary Ion Mass Spectrometry Imaging Mass Spectrometry and CARS Microspectroscopy Reveals Lipid Patterns Reminiscent of Gene Expression Patterns in the Wing Imaginal Disc of Drosophila melanogaster.
- Marty F Institute of Molecular Life Sciences, University of Zürich , Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
- Rago G FOM-Institute AMOLF , Science Park 104, 1098 XG Amsterdam, The Netherlands.
- Smith DF FOM-Institute AMOLF , Science Park 104, 1098 XG Amsterdam, The Netherlands.
- Gao X Institutional Mass Spectrometry Laboratory, The University of Texas Health Science Center at San Antonio , 8403 Floyd Curl Drive, MC-7760 San Antonio, Texas, United States.
- Eijkel GB FOM-Institute AMOLF , Science Park 104, 1098 XG Amsterdam, The Netherlands.
- MacAleese L FOM-Institute AMOLF , Science Park 104, 1098 XG Amsterdam, The Netherlands.
- Bonn M FOM-Institute AMOLF , Science Park 104, 1098 XG Amsterdam, The Netherlands.
- Brunner E Institute of Molecular Life Sciences, University of Zürich , Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
- Basler K Institute of Molecular Life Sciences, University of Zürich , Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
- Heeren RMA FOM-Institute AMOLF , Science Park 104, 1098 XG Amsterdam, The Netherlands.
- 2017-07-21
Published in:
- Analytical chemistry. - 2017
Animals
Drosophila melanogaster
Gene Expression Profiling
Glycerides
Imaginal Discs
Spectrometry, Mass, Secondary Ion
Spectrum Analysis, Raman
Time Factors
Wings, Animal
English
Using label-free ToF-SIMS imaging mass spectrometry, we generated a map of small molecules differentially expressed in the Drosophila wing imaginal disc. The distributions of these moieties were in line with gene expression patterns observed during wing imaginal disc development. Combining ToF-SIMS imaging and coherent anti-Stokes Raman spectroscopy (CARS) microspectroscopy allowed us to locally identify acylglycerols as the main constituents of the pattern differentiating the future body wall tissue from the wing blade tissue. The findings presented herein clearly demonstrate that lipid localization patterns are strongly correlated with a developmental gene expression. From this correlation, we hypothesize that lipids play a so far unrecognized role in organ development.
- Language
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- English
- Open access status
- hybrid
- Identifiers
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- DOI 10.1021/acs.analchem.7b00125
- PMID 28727418
- Persistent URL
- https://sonar.ch/global/documents/91779
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