Journal article
CONSTRUCTION AND EXPRESSION OF HYBRID PLASMINOGEN ACTIVATOR GENES
- Rajput, B CH-4002 Basel, Switzerland
- Alaimo, D Dept. of Biotechnology, CIBA-GEIGY Ltd., Switzerland
- Asselbergs, A M Dept. of Biotechnology, CIBA-GEIGY Ltd., Switzerland
- Reich, E CH-4002 Basel, Switzerland
- 2018-8-23
Published in:
- XIth International Congress on Thrombosis and Haemostasis. - Schattauer GmbH. - 1987
English
Hybrid plasminogen activator (PA) genes containing fragments of cDNA encoding the non-catalytic part of tissue-PA and the .catalytic domain of urokinase and vice versa were constructed and expressed in Chinese Hamster ovary (CHO) cells. The hybrid nature of the products in stably transformed cells was analyzed at the level of DNA and RNA using probes derived from different regions of the urokinase and tissue-PA cDNAs and at the protein level by means of polyclonal antibodies raised against tissue-PA and urokinase. The hybrid genes made hybrid RNAs and proteins of the expected sizes. The proteins were enzymatically active as determined by zymography and chromogenic enzyme assays and this activity was blocked by the appropriate antibodies. The effect on hybrid PAs of cyanogen bromide cleaved fibrinogen fragments, poly-D-lysine and heparin which are known to affect the activity of tissue-PA and urokinase differently was also studied
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- English
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- https://sonar.ch/global/documents/92635
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