Journal article
Pollen-derived nonallergenic substances enhance Th2-induced IgE production in B cells.
- Oeder S Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- Alessandrini F Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- Wirz OF Swiss Institute of Allergy and Asthma Research (SIAF), Davos, Switzerland.
- Braun A Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- Wimmer M Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- Frank U Christine Kühne - Center for Allergy Research and Education, CK-CARE, Davos, Switzerland.
- Hauser M Christine Kühne - Center for Allergy Research and Education, CK-CARE, Davos, Switzerland.
- Durner J Institute of Biochemical Plant Pathology, Helmholtz Center Munich, Neuherberg, Germany.
- Ferreira F Department of Molecular Biology, University of Salzburg, Salzburg, Austria.
- Ernst D Institute of Biochemical Plant Pathology, Helmholtz Center Munich, Neuherberg, Germany.
- Mempel M Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- Gilles S Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- Buters JT Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- Behrendt H Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- Traidl-Hoffmann C Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- Schmidt-Weber C Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- Akdis M Swiss Institute of Allergy and Asthma Research (SIAF), Davos, Switzerland.
- Gutermuth J Center of Allergy and Environment (ZAUM), Technische Universität München and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany.
- 2015-07-28
Published in:
- Allergy. - 2015
B cell
IgE
adjuvant
phytoprostane E1
pollen
Allergens
Ambrosia
Animals
Antibody Formation
Antigens, Plant
B-Lymphocytes
Female
Humans
Immunization
Immunoglobulin E
Immunologic Memory
Lymphocyte Activation
Mice
Ovalbumin
Plant Extracts
Pneumonia
Pollen
Th2 Cells
English
BACKGROUND
B cells play a central role in IgE-mediated allergies. In damaged airway epithelium, they are exposed directly to aeroallergens. We aimed to assess whether direct exposure of B cells to pollen constituents affects allergic sensitization.
METHODS
B cells from murine splenocytes and from blood samples of healthy donors were incubated for 8 days under Th2-like conditions with aqueous ragweed pollen extracts (Amb-APE) or its constituents. Secreted total IgM, IgG, and IgE was quantified by ELISA. Additionally, birch, grass, or pine-pollen extracts were tested. The number of viable cells was evaluated by ATP measurements. B-cell proliferation was measured by CFSE staining. IgE class switch was analyzed by quantitation of class switch transcripts. In an OVA/Alum i.p.-sensitization mouse model, Amb-APE was intranasally instilled for 11 consecutive days.
RESULTS
Upon Th2 priming of murine B cells, ragweed pollen extract caused a dose-dependent increase in IgE production, while IgG and IgM were not affected. The low-molecular-weight fraction and phytoprostane E1 (PPE1) increased IgE production, while Amb a 1 did not. PPE1 enhanced IgE also in human memory B cells. Under Th1 conditions, Amb-APE did not influence immunoglobulin secretion. The IgE elevation was not ragweed specific. It correlated with proliferation of viable B cells, but not with IgE class switch. In vivo, Amb-APE increased total IgE and showed adjuvant activity in allergic airway inflammation.
CONCLUSIONS
Aqueous pollen extracts, the protein-free fraction of Amb-APE, and the pollen-contained substance PPE1 specifically enhance IgE production in Th2-primed B cells. Thus, pollen-derived nonallergenic substances might be responsible for B-cell-dependent aggravation of IgE-mediated allergies.
B cells play a central role in IgE-mediated allergies. In damaged airway epithelium, they are exposed directly to aeroallergens. We aimed to assess whether direct exposure of B cells to pollen constituents affects allergic sensitization.
METHODS
B cells from murine splenocytes and from blood samples of healthy donors were incubated for 8 days under Th2-like conditions with aqueous ragweed pollen extracts (Amb-APE) or its constituents. Secreted total IgM, IgG, and IgE was quantified by ELISA. Additionally, birch, grass, or pine-pollen extracts were tested. The number of viable cells was evaluated by ATP measurements. B-cell proliferation was measured by CFSE staining. IgE class switch was analyzed by quantitation of class switch transcripts. In an OVA/Alum i.p.-sensitization mouse model, Amb-APE was intranasally instilled for 11 consecutive days.
RESULTS
Upon Th2 priming of murine B cells, ragweed pollen extract caused a dose-dependent increase in IgE production, while IgG and IgM were not affected. The low-molecular-weight fraction and phytoprostane E1 (PPE1) increased IgE production, while Amb a 1 did not. PPE1 enhanced IgE also in human memory B cells. Under Th1 conditions, Amb-APE did not influence immunoglobulin secretion. The IgE elevation was not ragweed specific. It correlated with proliferation of viable B cells, but not with IgE class switch. In vivo, Amb-APE increased total IgE and showed adjuvant activity in allergic airway inflammation.
CONCLUSIONS
Aqueous pollen extracts, the protein-free fraction of Amb-APE, and the pollen-contained substance PPE1 specifically enhance IgE production in Th2-primed B cells. Thus, pollen-derived nonallergenic substances might be responsible for B-cell-dependent aggravation of IgE-mediated allergies.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1111/all.12707
- PMID 26214762
- Persistent URL
- https://sonar.ch/global/documents/93211
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