Journal article
A new protocol for improving efficiency of autologous peripheral blood stem cell collection in patients with high white blood cell counts.
- Boehlen R Blood Transfusion Center Aarau, Swiss Red Cross, Kantonsspital Aarau AG, Aarau, Switzerland.
- Fernandez P Institute of Laboratory Medicine, Kantonsspital Aarau AG, Aarau, Switzerland.
- Bargetzi M Division of Hematology, University Medical Clinic, Kantonsspital Aarau AG, Aarau, Switzerland.
- Sigle JP Blood Transfusion Center Aarau, Swiss Red Cross, Kantonsspital Aarau AG, Aarau, Switzerland.
- 2020-04-19
Published in:
- Journal of clinical apheresis. - 2020
English
BACKGROUND
Collection efficiency (CE) of peripheral blood stem cell (PBSC) collections is negatively affected by increasing white blood cell (WBC) counts of the patient. This study compared a new optimized mononuclear cell (MNC) collection protocol (OPP) to the standard MNC collection protocol recommended by the manufacturer (STP) for PBSC collection in patients with WBC counts >35 000/μL.
STUDY DESIGN AND METHODS
Single-center, retrospective, and observational study of 81 autologous PBSC collections on Fenwal Amicus cell separators in 70 adult patients.
RESULTS
Median peripheral WBC count (×103 /μL; 44.2 in OPP group vs 46.5 in STP group) and median CD34+ count (105/μL in OPP group vs 40/μL in STP group) at the beginning of PBSC collection did not differ significantly. Median CE2 (45% vs 31%; P < .001) as well as CD34+ yield of the apheresis product both with regards to median absolute CD34+ content (×106 ; 793 vs 188; P = .001) as well as median CD34+ content (×106 )/kg body weight (8.93 vs 2.51; P = .002) were significantly higher for the OPP. Overall, 18/21 (86%) patients with the OPP obtained their target CD34+ amount with a single apheresis session, compared to 25/50 (50%) with the STP (P = .005). PBSC collections using OPP lasted significantly longer (median 377 minutes vs 260 minutes; P < .001) than with the STP.
CONCLUSIONS
The OPP significantly improves CE2 for PBSC collections on Fenwal Amicus cell separators in patients with pre-apheresis WBC counts >35 000/μL and significantly reduces the necessity for multiple apheresis sessions. The OPP is therefore suited to reduce both patient burden and cost in autologous PBSC collection.
Collection efficiency (CE) of peripheral blood stem cell (PBSC) collections is negatively affected by increasing white blood cell (WBC) counts of the patient. This study compared a new optimized mononuclear cell (MNC) collection protocol (OPP) to the standard MNC collection protocol recommended by the manufacturer (STP) for PBSC collection in patients with WBC counts >35 000/μL.
STUDY DESIGN AND METHODS
Single-center, retrospective, and observational study of 81 autologous PBSC collections on Fenwal Amicus cell separators in 70 adult patients.
RESULTS
Median peripheral WBC count (×103 /μL; 44.2 in OPP group vs 46.5 in STP group) and median CD34+ count (105/μL in OPP group vs 40/μL in STP group) at the beginning of PBSC collection did not differ significantly. Median CE2 (45% vs 31%; P < .001) as well as CD34+ yield of the apheresis product both with regards to median absolute CD34+ content (×106 ; 793 vs 188; P = .001) as well as median CD34+ content (×106 )/kg body weight (8.93 vs 2.51; P = .002) were significantly higher for the OPP. Overall, 18/21 (86%) patients with the OPP obtained their target CD34+ amount with a single apheresis session, compared to 25/50 (50%) with the STP (P = .005). PBSC collections using OPP lasted significantly longer (median 377 minutes vs 260 minutes; P < .001) than with the STP.
CONCLUSIONS
The OPP significantly improves CE2 for PBSC collections on Fenwal Amicus cell separators in patients with pre-apheresis WBC counts >35 000/μL and significantly reduces the necessity for multiple apheresis sessions. The OPP is therefore suited to reduce both patient burden and cost in autologous PBSC collection.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1002/jca.21782
- PMID 32304598
- Persistent URL
- https://sonar.ch/global/documents/93354
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