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Geometric cues forecast the switch from two- to three-dimensional growth in Physcomitrella patens.

  • Tang H Laboratory of Plant Developmental Biology, Wageningen University & Research, 6708 PB, Wageningen, the Netherlands.
  • Duijts K Laboratory of Cell Biology, Wageningen University & Research, 6708 PE, Wageningen, the Netherlands.
  • Bezanilla M Biological Sciences Department, Dartmouth College, Hanover, NH, 03755, USA.
  • Scheres B Laboratory of Plant Developmental Biology, Wageningen University & Research, 6708 PB, Wageningen, the Netherlands.
  • Vermeer JEM Laboratory of Cell and Molecular Biology, Institute of Biology, University of Neuchâtel, 2000, Neuchâtel, Switzerland.
  • Willemsen V Laboratory of Plant Developmental Biology, Wageningen University & Research, 6708 PB, Wageningen, the Netherlands.
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  • 2019-10-23
Published in:
  • The New phytologist. - 2020
Physcomitrella patens
2D-to-3D development
asymmetric cell division
cell fate switch
geometric cues
English During land colonization, plants acquired a range of body plan adaptations, of which the innovation of three-dimensional (3D) tissues increased organismal complexity and reproductivity. In the moss, Physcomitrella patens, a 3D leafy gametophore originates from filamentous cells that grow in a two-dimensional (2D) plane through a series of asymmetric cell divisions. Asymmetric cell divisions that coincide with different cell division planes and growth directions enable the developmental switch from 2D to 3D, but insights into the underlying mechanisms coordinating this switch are still incomplete. Using 2D and 3D imaging and image segmentation, we characterized two geometric cues, the width of the initial cell and the angle of the transition division plane, which sufficiently distinguished a gametophore initial cell from a branch initial cell. These identified cues were further confirmed in gametophore formation mutants. The identification of a fluorescent marker allowed us to successfully predict the gametophore initial cell with > 90% accuracy before morphological changes, supporting our hypothesis that, before the transition division, parental cells of the gametophore initials possess different properties from those of the branch initials. Our results suggest that the cell fate decision of the initial cell is determined in the parental cell, before the transition division.
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  • English
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https://sonar.ch/global/documents/93399
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