Back
In situ structure determination by subtomogram averaging.
Journal article

In situ structure determination by subtomogram averaging.

  • Castaño-Díez D BioEM Lab, Center for Cellular Imaging and Nanoanalytics, Biozentrum, University of Basel, Mattenstrasse 26, CH-4058, Basel, Switzerland. Electronic address: daniel.castano@unibas.ch.
  • Zanetti G Institute of Structural and Molecular Biology, Birkbeck College, Malet St., London, WC1E 7HX, UK. Electronic address: g.zanetti@mail.cryst.bbk.ac.uk.
  • 2019-06-25
Published in:
  • Current opinion in structural biology. - 2019
Cryoelectron Microscopy
Electron Microscope Tomography
Image Processing, Computer-Assisted
English Cryo-tomography and subtomogram averaging are increasingly popular techniques for structural determination of macromolecular complexes in situ. They have the potential to achieve high-resolution views of native complexes, together with the details of their location relative to interacting molecules. The subtomogram averaging (StA) pipelines are well-established, with current developments aiming to optimise each step by reducing manual intervention and user decisions, following similar trends in single-particle approaches that have dramatically increased their popularity. Here, we review the main steps of typical StA workflows. We focus on considerations arising from the fact that the objects of study are embedded within unique crowded environments, and we emphasise those steps where careful decisions need to be made by the user.
Language
  • English
Open access status
hybrid
Identifiers
Persistent URL
https://sonar.ch/global/documents/93539
Statistics
Document views: 80 File downloads: