Back
CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila.
Journal article

CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila.

  • Fritsch C Department of Biology, University of Fribourg, Fribourg, Switzerland. cornelia.fritsch@unifr.ch.
  • Sprecher SG Department of Biology, University of Fribourg, Fribourg, Switzerland.
  • 2019-09-26
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2020
Brain
CRISPR
Conditional alleles
Drosophila
Genome editing
Alleles
Animals
Brain
CRISPR-Cas Systems
Drosophila
Drosophila melanogaster
Gene Editing
Models, Genetic
Plasmids
Polymerase Chain Reaction
English Continuous implementation of new techniques allowing increasingly precise genetic manipulations makes the fruit fly Drosophila melanogaster an impacting model to study the nervous system. While transgenic approaches have been heavily used to investigate how the brain develops, genome editing has been notoriously hard in the fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to replace the endogenous genome sequence with a designed template of interest. We here provide a protocol to generate an FRT/FLP-based conditional GFP or HA-flagged gene knockout.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/93565
Statistics
Document views: 29 File downloads: