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Characterization of Protein Conformational Changes with Sparse Spin-Label Distance Constraints.
Journal article

Characterization of Protein Conformational Changes with Sparse Spin-Label Distance Constraints.

  • Jeschke G Lab. Phys. Chem., ETH Zürich, Wolfgang-Pauli-Strasse 10, CH-8093 Zürich, Switzerland.
  • 2015-11-24
Published in:
  • Journal of chemical theory and computation. - 2012
English The combination of site-directed spin labeling with pulse EPR distance measurements can provide a moderate number of distance constraints on the nanometer length scale for proteins in different states. By adapting an existing algorithm (Zheng, W.; Brooks, B. R. Biophys. J. 2006, 90, 4327) to the problem, we address the question to what extent conformational change can be characterized when the protein structure is known for one of the states, whereas only a sparse set of distance constraints between spin labels is available for the other state. We find that the type and general direction of the conformational change can be recognized, while the amplitude may be uncertain.
Language
  • English
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closed
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Persistent URL
https://sonar.ch/global/documents/93760
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