Journal article
Silver-nanoparticles increase bactericidal activity and radical oxygen responses against bacterial pathogens in human osteoclasts.
- Aurore V Anatomy unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
- Caldana F Anatomy unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
- Blanchard M Anatomy unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
- Kharoubi Hess S Anatomy unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
- Lannes N Anatomy unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
- Mantel PY Anatomy unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
- Filgueira L Anatomy unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
- Walch M Anatomy unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland. Electronic address: michael.walch@unifr.ch.
- 2017-11-21
Published in:
- Nanomedicine : nanotechnology, biology, and medicine. - 2018
Antibacterial activity
Bone infection
Monocyte-derived osteoclasts
Reactive oxygen species
Silver-nanoparticle
Anti-Bacterial Agents
Cells, Cultured
Humans
Metal Nanoparticles
Osteoclasts
Phagocytosis
Reactive Oxygen Species
Silver
Staphylococcal Infections
Staphylococcus aureus
English
Bone infections are difficult to treat and can lead to severe tissue destruction. Acute bone infections are usually caused by Staphylococcus aureus. Osteoclasts, which belong to the monocyte/macrophage lineage, are the key cells in bone infections. They are not well equipped for killing bacteria and may serve as a reservoir for bacterial pathogens. Silver has been known for centuries for its bactericidal activity. Here, we investigated the bactericidal effects of nano-silver particles in bacteria infected human osteoclasts. We found that nano-silver in per se non-toxic concentration enhanced the bactericidal activity in osteoclasts against intracellular Methicillin-resistant, virulent Staphylococcus aureus. The reduced bacterial survival in nano-silver pretreated cells correlated with increased reactive oxygen responses towards the invading pathogens. Overall, these results indicate that nano-silver compounds should be considered as an effective treatment and prevention option for bacterial bone and orthopedic implant infections.
- Language
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- English
- Open access status
- hybrid
- Identifiers
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- DOI 10.1016/j.nano.2017.11.006
- PMID 29155361
- Persistent URL
- https://sonar.ch/global/documents/93792
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