Back
Posttranslational modifications of trichomonad tubulins; identification of multiple glutamylation sites.
Journal article

Posttranslational modifications of trichomonad tubulins; identification of multiple glutamylation sites.

  • 1998-07-14
Published in:
  • FEBS letters. - 1998
Amino Acid Sequence
Animals
Cytoskeleton
Eukaryotic Cells
Glutamic Acid
Mass Spectrometry
Molecular Sequence Data
Peptide Fragments
Protein Processing, Post-Translational
Sequence Analysis
Trichomonas
Tubulin
English The alpha- and beta-tubulins present in cytoskeletons of Tritrichomonas mobilensis are extensively glutamylated. Automated sequencing and mass spectrometry of the carboxyterminal peptides identifies 4 glutamylation sites in alpha- and 2 sites in beta-tubulin. They are marked by asterisks in the terminal sequences GDE*E*E*E*DDG (alpha) and EGE*E*DEEAEA (beta). This is the first report that tubulin glutamylation can occur at multiple sites. Although T. mobilensis has four flagellae the tubulins lack polyglycylation. Thus glycylation is not necessary for formation or function of axonemal microtubules. Alpha-tubulin is completely acetylated at lysine 40 and shows no tyrosine cycle. Peptide sequences establish two distinct beta-tubulins.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/93832
Statistics
Document views: 18 File downloads: