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Aluminium foil as a single-use substrate for MALDI-MS fingerprinting of different melanoma cell lines.

  • Bondarenko A Laboratoire d'Electrochimie Physique et Analytique, École Polytechnique Fédérale de Lausanne, EPFL Valais Wallis, CH-1951 Sion, Switzerland. hubert.girault@epfl.ch.
  • Zhu Y Laboratoire d'Electrochimie Physique et Analytique, École Polytechnique Fédérale de Lausanne, EPFL Valais Wallis, CH-1951 Sion, Switzerland. hubert.girault@epfl.ch.
  • Qiao L Laboratoire d'Electrochimie Physique et Analytique, École Polytechnique Fédérale de Lausanne, EPFL Valais Wallis, CH-1951 Sion, Switzerland. hubert.girault@epfl.ch.
  • Cortés Salazar F Laboratoire d'Electrochimie Physique et Analytique, École Polytechnique Fédérale de Lausanne, EPFL Valais Wallis, CH-1951 Sion, Switzerland. hubert.girault@epfl.ch.
  • Pick H Laboratoire de Chimie Physique des Polymères et Membranes, École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
  • Girault HH Laboratoire d'Electrochimie Physique et Analytique, École Polytechnique Fédérale de Lausanne, EPFL Valais Wallis, CH-1951 Sion, Switzerland. hubert.girault@epfl.ch.
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  • 2016-05-04
Published in:
  • The Analyst. - 2016
Aluminum
Cell Line
Humans
Melanoma
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
English Herein, we present the intact cell matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the fingerprinting of human melanoma cancer cell lines grown on aluminium foil. To perform the MALDI-MS assay, melanoma cells were cultured on a flat and thin foil, which was directly transferred to the target plate of MALDI-MS for analysis. The influence of a wide range of cell fixation protocols (i.e. formalin-based and alcohol-based methods) and MALDI matrices on the obtained characteristic spectra was investigated. For the optimization of the MALDI-MS protocol, the MS fingerprints of the melanoma WM-239 cell line with and without an overexpressed enhanced green fluorescent protein were employed. The fingerprints obtained from WM-239 cells grown on aluminium foil were compared with the intact cell MALDI-MS of the cell pellet and presented higher sensitivity in a high m/z range. The optimized protocol was subsequently applied to characterise melanoma cell lines derived from different cancer stages and allowed identification of unique MS signals that could be used for differentiation between the studied cell lines (i.e. molecular weight equal to 10.0 kDa and 26.1 kDa).
Language
  • English
Open access status
green
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https://sonar.ch/global/documents/96988
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