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Some like it sticky: targeting of the rice gene Waxy.
Journal article

Some like it sticky: targeting of the rice gene Waxy.

  • Hohn B Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland. barbara.hohn@fmi.ch
  • Puchta H
  • 2003-02-25
Published in:
  • Trends in plant science. - 2003
Gene Deletion
Gene Targeting
Genes, Plant
Oryza
Plant Proteins
Reproducibility of Results
Starch Synthase
English For the first time, a gene function can be knocked out by homologous recombination in a monocot plant species. Fertile rice plants with the desired change in their genome can be obtained reproducibly. Although the absolute frequency of targeting does not exceed that of previous experiments in dicots, the use of a negative selectable marker at both extremities of the targeting construct reduces the amount of screening and excludes ectopic targeting, an unwanted by-product of gene targeting.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/97522
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