Assessment of cell proliferation with resazurin-based fluorescent dye.

Czekanska EM

doi:10.1007/978-1-61779-108-6_5

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Journal article

Assessment of cell proliferation with resazurin-based fluorescent dye.

Czekanska EM AO Research Institute, Davos Platz, Switzerland. ewa.czekanska@aofoundation.org

2011-04-07

Published in:

Methods in molecular biology (Clifton, N.J.). - 2011

English The Alamar Blue assay is based on enzymatic reduction of indicator dye by viable cells and serves as an effective tool for assessing cell proliferation and as a screening technique. It can be applied in studies concentrating on animal, plant, yeast, and bacteria cells. Among the various methods for cell viability and cytotoxicity, it utilises all features of ideal and reliable test; it is one-step, sensitive, safe, non-toxic for cells, and cost-effective.

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