Journal article
Nogo in the Mammalian cochlea.
- 2009-06-24
Published in:
- Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology. - 2009
Acoustic Stimulation
Animals
Audiometry
Auditory Threshold
Cochlea
Fluorescent Antibody Technique
Hair Cells, Vestibular
Hearing Loss
Immunohistochemistry
Mammals
Mice
Mice, Inbred C57BL
Mice, Knockout
Myelin Proteins
Nogo Proteins
Organ Culture Techniques
RNA
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
English
HYPOTHESIS
Different members of the Nogo system are expressed in the mammalian cochlea.
BACKGROUND
The protein Nogo has gained a lot of attention during the last couple of years because it inhibits neurite outgrowth in the adult central nervous system. In contrast to the central nervous system, very little is known regarding the expression and possible function of the Nogo system within the inner ear.
METHODS
Using reverse-transcriptase-polymerase chain reaction and immunohistochemistry, we analyzed for the expression of members of the Nogo system within the cochlea. In addition, we determined hearing levels of Nogo A knockout and wild-type mice with auditory brainstem response audiometry.
RESULTS
In this study, we demonstrate the expression of Nogo A, B, C, and of Nogo receptor mRNA in the organ of Corti, spiral ganglion, and stria vascularis. Immunohistochemistry revealed that Nogo A and Nogo receptor localize to the spiral ganglion neurons. Interestingly, Nogo A expression was also observed in the outer and inner hair cells of the organ of Corti. As revealed by light microscopy, deletion of Nogo A does not alter cochlear microanatomy. We have assessed hearing levels in 10-month old wild-type and Nogo A knockout mice, and thereby, we could not detect any differences between these 2 groups.
CONCLUSION
Different members of the Nogo family are expressed in the mammalian cochlea. Deletion of Nogo A does not alter cochlea microanatomy or hearing levels compared with wild-type mice.
Different members of the Nogo system are expressed in the mammalian cochlea.
BACKGROUND
The protein Nogo has gained a lot of attention during the last couple of years because it inhibits neurite outgrowth in the adult central nervous system. In contrast to the central nervous system, very little is known regarding the expression and possible function of the Nogo system within the inner ear.
METHODS
Using reverse-transcriptase-polymerase chain reaction and immunohistochemistry, we analyzed for the expression of members of the Nogo system within the cochlea. In addition, we determined hearing levels of Nogo A knockout and wild-type mice with auditory brainstem response audiometry.
RESULTS
In this study, we demonstrate the expression of Nogo A, B, C, and of Nogo receptor mRNA in the organ of Corti, spiral ganglion, and stria vascularis. Immunohistochemistry revealed that Nogo A and Nogo receptor localize to the spiral ganglion neurons. Interestingly, Nogo A expression was also observed in the outer and inner hair cells of the organ of Corti. As revealed by light microscopy, deletion of Nogo A does not alter cochlear microanatomy. We have assessed hearing levels in 10-month old wild-type and Nogo A knockout mice, and thereby, we could not detect any differences between these 2 groups.
CONCLUSION
Different members of the Nogo family are expressed in the mammalian cochlea. Deletion of Nogo A does not alter cochlea microanatomy or hearing levels compared with wild-type mice.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1097/MAO.0b013e3181ab95c5
- PMID 19546827
- Persistent URL
- https://sonar.ch/global/documents/98215
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