IL-12 administration leads to a transient depletion of T cells, B cells, and APCs and concomitant abrogation of the HLA-A2.1-restricted CTL response in transgenic mice.
- Peter K Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
- Brunda MJ
- Corradin G
- 2002-06-22
Published in:
- Journal of immunology (Baltimore, Md. : 1950). - 2002
AIDS Vaccines
Animals
Antigen-Presenting Cells
B-Lymphocytes
Cells, Cultured
Cytotoxicity, Immunologic
Down-Regulation
Epitopes, T-Lymphocyte
Female
H-2 Antigens
HLA-A2 Antigen
Immunodominant Epitopes
Injections, Intraperitoneal
Interleukin-12
Lymphocyte Depletion
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Transgenic
Oligopeptides
Recombinant Proteins
Spleen
T-Lymphocytes
English
The injection of a mixture of bona fide T cell epitopes can lead to the occurrence of immunodominance, meaning that the immune response is focused on the recognition of a single epitope or a small portion of the epitopes injected. We have previously demonstrated that the administration of rIL-12 can counteract immunodominance in BALB/c mice. In this study, we show that the administration of rIL-12 to HLA-A2.1 transgenic mice (A2k(b) mice) abrogates specifically the immune response against HLA-A2.1-restricted HIV epitopes in the spleen. This lack of immune response is most probably due to a transient depletion of B cells, T cells, macrophages, and dendritic cells in this organ. Therefore, our study explains the mechanism of immunosuppression by rIL-12 in vivo.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.4049/jimmunol.169.1.63
- PMID 12077229
- Persistent URL
- https://sonar.ch/global/documents/98648
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