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Journal article

Resolution and enhancement in nanoantenna-based fluorescence microscopy.

  • 2009-11-06
Published in:
  • Nano letters. - 2009
Computer Simulation
Contrast Media
Gold
Image Enhancement
Image Interpretation, Computer-Assisted
Microscopy, Fluorescence
Models, Chemical
Nanostructures
Reproducibility of Results
Sensitivity and Specificity
English Single gold nanoparticles can act as nanoantennas for enhancing the fluorescence of emitters in their near fields. Here we present experimental and theoretical studies of scanning antenna-based fluorescence microscopy as a function of the diameter of the gold nanoparticle. We examine the interplay between fluorescence enhancement and spatial resolution and discuss the requirements for deciphering single molecules in a dense sample. Resolutions better than 20 nm and fluorescence enhancement up to 30 times are demonstrated experimentally. By accounting for the tip shaft and the sample interface in finite-difference time-domain calculations, we explain why the measured fluorescence enhancements are higher in the presence of an interface than the values predicted for a homogeneous environment.
Language
  • English
Open access status
green
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Persistent URL
https://sonar.ch/global/documents/98870
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