Label-free imaging of cerebral β-amyloidosis with extended-focus optical coherence microscopy.
- Bolmont T Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.
- Bouwens A
- Pache C
- Dimitrov M
- Berclaz C
- Villiger M
- Wegenast-Braun BM
- Lasser T
- Fraering PC
- 2012-10-19
Published in:
- The Journal of neuroscience : the official journal of the Society for Neuroscience. - 2012
Alzheimer Disease
Amyloid beta-Peptides
Animals
Cerebral Amyloid Angiopathy
Disease Models, Animal
Fourier Analysis
Humans
Mice
Mice, Transgenic
Microscopy, Confocal
Neuroimaging
Plaque, Amyloid
Staining and Labeling
Tomography, Optical Coherence
English
We demonstrate label-free imaging of cerebral β-amyloidosis ex vivo and in a living mouse model of Alzheimer's disease using extended-focus Fourier domain optical coherence microscopy (xfOCM). xfOCM provides 3D, high-resolution images of individual β-amyloid plaques in the brain parenchyma and vasculature and requires no staining of the alzheimeric sample under investigation. xfOCM also opens the possibility to perform minimally invasive studies of β-amyloid pathology in vivo, without the use of labeling methods, which potentially confound experimental findings.
- Language
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- English
- Open access status
- hybrid
- Identifiers
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- DOI 10.1523/JNEUROSCI.0925-12.2012
- PMID 23077040
- Persistent URL
- https://sonar.ch/global/documents/99392
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