Photoactivation of silicon rhodamines via a light-induced protonation.
- Frei MS Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany.
- Hoess P Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany.
- Lampe M Advanced Light Microscopy Facility (ALMF), European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany.
- Nijmeijer B Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany.
- Kueblbeck M Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany.
- Ellenberg J Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany.
- Wadepohl H Anorganisch-Chemisches Institut, University of Heidelberg, Im Neuenheimer Feld 270, 69120, Heidelberg, Germany.
- Ries J Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany.
- Pitsch S Spirochrome AG, Chalberweidstrasse 4, CH-8260, Stein am Rhein, Switzerland.
- Reymond L Biomolecular Screening Facility, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland. luc.reymond@epfl.ch.
- Johnsson K Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany. johnsson@mr.mpg.de.
- 2019-10-10
Published in:
- Nature communications. - 2019
Animals
COS Cells
Chlorocebus aethiops
Fluorescent Dyes
HeLa Cells
Humans
Intravital Microscopy
Light
Microscopy, Fluorescence
Photochemical Processes
Protons
Rhodamines
Silicon
Single Molecule Imaging
English
Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore's outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.
- Language
-
- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41467-019-12480-3
- PMID 31594948
- Persistent URL
- https://sonar.ch/global/documents/99778
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