The Role of Tricellulin in Epithelial Jamming and Unjamming via Segmentation of Tricellular Junctions.
- Lohmann S Laboratory of Thermodynamics in Emerging Technologies ETH Zurich Zurich 8092 Switzerland.
- Giampietro C EMPA Swiss Federal Laboratories for Materials Science and Technology Experimental Continuum Mechanics Dübendorf 8600 Switzerland.
- Pramotton FM Laboratory of Thermodynamics in Emerging Technologies ETH Zurich Zurich 8092 Switzerland.
- Al-Nuaimi D Laboratory of Thermodynamics in Emerging Technologies ETH Zurich Zurich 8092 Switzerland.
- Poli A IFOM- The FIRC Institute of Molecular Oncology Spatiotemporal organization of the nucleus Unit Milan 20139 Italy.
- Maiuri P IFOM- The FIRC Institute of Molecular Oncology Spatiotemporal organization of the nucleus Unit Milan 20139 Italy.
- Poulikakos D Laboratory of Thermodynamics in Emerging Technologies ETH Zurich Zurich 8092 Switzerland.
- Ferrari A Laboratory of Thermodynamics in Emerging Technologies ETH Zurich Zurich 8092 Switzerland.
- 2020-08-11
Published in:
- Advanced science (Weinheim, Baden-Wurttemberg, Germany). - 2020
English
Collective cellular behavior in confluent monolayers supports physiological and pathological processes of epithelial development, regeneration, and carcinogenesis. Here, the attainment of a mature and static tissue configuration or the local reactivation of cell motility involve a dynamic regulation of the junctions established between neighboring cells. Tricellular junctions (tTJs), established at vertexes where three cells meet, are ideally located to control cellular shape and coordinate multicellular movements. However, their function in epithelial tissue dynamic remains poorly defined. To investigate the role of tTJs establishment and maturation in the jamming and unjamming transitions of epithelial monolayers, a semi-automatic image-processing pipeline is developed and validated enabling the unbiased and spatially resolved determination of the tTJ maturity state based on the localization of fluorescent reporters. The software resolves the variation of tTJ maturity accompanying collective transitions during tissue maturation, wound healing, and upon the adaptation to osmolarity changes. Altogether, this work establishes junctional maturity at tricellular contacts as a novel biological descriptor of collective responses in epithelial monolayers.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1002/advs.202001213
- PMID 32775171
- Persistent URL
- https://sonar.ch/global/documents/99806
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