In Vitro Study of ISApl1-Mediated Mobilization of the Colistin Resistance Gene mcr-1

Poirel, Laurent; Kieffer, Nicolas; Nordmann, Patrice

doi:10.1128/aac.00127-17

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Journal article

In Vitro Study of ISApl1-Mediated Mobilization of the Colistin Resistance Gene mcr-1

Poirel, Laurent Swiss National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland
Kieffer, Nicolas Swiss National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland
Nordmann, Patrice University of Lausanne and University Hospital Center, Lausanne, Switzerland

2017-6-27

Published in:

Antimicrobial Agents and Chemotherapy. - American Society for Microbiology. - 2017, vol. 61, no. 7

English ABSTRACT
The plasmid-mediated mcr-1 gene encodes a phosphoethanolamine transferase that confers resistance to polymyxins. The mcr-1 gene is associated with insertion sequence ISApl1 (IS30 family). In vitro mobilization assays demonstrated the functionality of the composite transposon structure ISApl1-mcr-1-ISApl1. Transposition generated a 2-bp duplication and occurred in AT-rich DNA regions. This is the first report demonstrating the mobility of the mcr-1 gene by transposition.

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