Effects of endotoxin and catecholamines on hepatic mitochondrial respiration.
- Porta F Department of Intensive Care Medicine, Inselspital, Bern University Hospital and University of Bern, Bern, Switzerland.
- Bracht H
- Weikert C
- Beck M
- Takala J
- Brandt S
- Hiltebrand LB
- Jakob SM
- 2009-07-16
Published in:
- Inflammation. - 2009
Adenosine Diphosphate
Adenosine Triphosphate
Animals
Catecholamines
Cell Respiration
Endotoxins
Mitochondria, Liver
Oxygen Consumption
Swine
Time Factors
English
Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.
- Language
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- English
- Open access status
- green
- Identifiers
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- DOI 10.1007/s10753-009-9138-y
- PMID 19603262
- Persistent URL
- https://sonar.ch/global/documents/1196
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