Effects of endotoxin and catecholamines on hepatic mitochondrial respiration.

Porta F; Bracht H; Weikert C; Beck M; Takala J; Brandt S; Hiltebrand LB; Jakob SM

doi:10.1007/s10753-009-9138-y

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Journal article

Effects of endotoxin and catecholamines on hepatic mitochondrial respiration.

Porta F Department of Intensive Care Medicine, Inselspital, Bern University Hospital and University of Bern, Bern, Switzerland.
Bracht H
Weikert C
Beck M
Takala J
Brandt S
Hiltebrand LB
Jakob SM

2009-07-16

Published in:

Inflammation. - 2009

English Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.

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