Journal article
DNA at the entry-exit of the nucleosome observed by cryoelectron microscopy.
- Furrer P Laboratoire d'Analyse Ultrastructurale, Section de Biologie, Université de Lausanne, Dorigny, Switzerland.
- Bednar J
- Dubochet J
- Hamiche A
- Prunell A
- 1995-05-01
Published in:
- Journal of structural biology. - 1995
Animals
Chromatin
Computer Graphics
Crystallography, X-Ray
DNA
Electrophoresis, Polyacrylamide Gel
Freezing
Histones
Image Processing, Computer-Assisted
Microscopy, Electron
Models, Molecular
Nucleic Acid Conformation
Nucleosomes
English
Whereas the DNA path inside the nucleosome is well established, it is essentially unknown in the "entry-exit" region, a missing piece in our understanding of the chromatin fiber's folding. The three-dimensional structure of "linker" DNA was investigated here on single nucleosomes reconstituted without H1 on a 256-bp DNA fragment. Cryoelectron microscopy and three-dimensional reconstruction of the DNA axis reveal these nucleosomes as 1.61 +/- 0.15 left-handed superhelical turn particles whose DNA arms bend away from the core particle, thus preventing the occurrence of a crossing in the entry-exit region.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1006/jsbi.1995.1017
- PMID 7662486
- Persistent URL
- https://sonar.ch/global/documents/130572
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