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Nanopore Diameters Tune Strain in Extruded Fibronectin Fibers.
Journal article

Nanopore Diameters Tune Strain in Extruded Fibronectin Fibers.

  • Raoufi M Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems , Heisenbergstraße 3, D-70569 Stuttgart, Germany.
  • Das T Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems , Heisenbergstraße 3, D-70569 Stuttgart, Germany.
  • Schoen I Laboratory of Applied Mechanobiology, Department of Health Sciences and Technology ETH Zurich , Vladimir-Prelog Weg 4 (HCI F443), CH-8093 Zurich, Switzerland.
  • Vogel V Laboratory of Applied Mechanobiology, Department of Health Sciences and Technology ETH Zurich , Vladimir-Prelog Weg 4 (HCI F443), CH-8093 Zurich, Switzerland.
  • Brüggemann D Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems , Heisenbergstraße 3, D-70569 Stuttgart, Germany.
  • Spatz JP Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems , Heisenbergstraße 3, D-70569 Stuttgart, Germany.
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  • 2015-09-12
Published in:
  • Nano letters. - 2015
AAO template
FRET
conformational changes
extrusion
protein nanofiber
Fibronectins
Fluorescence Resonance Energy Transfer
Microscopy, Electron, Scanning
Nanopores
English Fibronectin is present in the extracellular matrix and can be assembled into nanofibers in vivo by undergoing conformational changes. Here, we present a novel approach to prepare fibronectin nanofibers under physiological conditions using an extrusion approach through nanoporous aluminum oxide membranes. This one-step process can prepare nanofiber bundles up to a millimeter in length and with uniform fiber diameters in the nanometer range. Most importantly, by using different pore diameters and protein concentrations in the extrusion process, we could induce varying lasting structural changes in the fibers, which were monitored by Förster resonance energy transfer and should impose different physiological functions.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/139882
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