Efficient electroporation of peptides into adherent cells: investigation of the role of mechano-growth factor in chondrocyte culture.

Schönenberger C; Schütz A; Franco-Obregón A; Zenobi-Wong M

doi:10.1007/s10529-010-0508-1

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Efficient electroporation of peptides into adherent cells: investigation of the role of mechano-growth factor in chondrocyte culture.

Schönenberger C Laboratory for Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETZ F84, ETH Zürich, Gloriastrasse 35, 8092 Zurich, Switzerland.
Schütz A
Franco-Obregón A
Zenobi-Wong M

2011-01-06

Published in:

Biotechnology letters. - 2011

English Peptide therapeutics are of increasing interest due to their biological specificity. We used a simple technique to study the efficacy of inducing peptides into adherent chondrocytes by transiently permeabilizing the membrane with electric pulses (in situ electroporation). Mechano-growth factor (MGF) was selected as a model peptide. FITC-labeled MGF was added to cultures of adherent primary chondrocytes grown on ITO coated glass slides. Cells were subjected to 3-9 pulses of 175-275 V and evaluated by flow cytometry. Under optimal conditions, an electroporation efficiency of close to 50% could be achieved. This technique can be used to study the functional domains of intracellular peptides, peptide inhibition of signal transduction and intracrine-mediated effects of peptides in adherent cells.

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