Efficient electroporation of peptides into adherent cells: investigation of the role of mechano-growth factor in chondrocyte culture.
- Schönenberger C Laboratory for Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETZ F84, ETH Zürich, Gloriastrasse 35, 8092 Zurich, Switzerland.
- Schütz A
- Franco-Obregón A
- Zenobi-Wong M
- 2011-01-06
Published in:
- Biotechnology letters. - 2011
Animals
Cattle
Cell Adhesion
Cells, Cultured
Chondrocytes
Electroporation
Fluorescein-5-isothiocyanate
Insulin-Like Growth Factor I
Peptides
Staining and Labeling
English
Peptide therapeutics are of increasing interest due to their biological specificity. We used a simple technique to study the efficacy of inducing peptides into adherent chondrocytes by transiently permeabilizing the membrane with electric pulses (in situ electroporation). Mechano-growth factor (MGF) was selected as a model peptide. FITC-labeled MGF was added to cultures of adherent primary chondrocytes grown on ITO coated glass slides. Cells were subjected to 3-9 pulses of 175-275 V and evaluated by flow cytometry. Under optimal conditions, an electroporation efficiency of close to 50% could be achieved. This technique can be used to study the functional domains of intracellular peptides, peptide inhibition of signal transduction and intracrine-mediated effects of peptides in adherent cells.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1007/s10529-010-0508-1
- PMID 21207114
- Persistent URL
- https://sonar.ch/global/documents/143217
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