Antiseptic solutions modulate the paracrine-like activity of bone chips: differential impact of chlorhexidine and sodium hypochlorite.
- Sawada K Department of Cranio Maxillofacial Surgery, Inselspital, University of Bern, Bern, Switzerland.
- Caballé-Serrano J Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland.
- Bosshardt DD Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland.
- Schaller B Department of Cranio Maxillofacial Surgery, Inselspital, University of Bern, Bern, Switzerland.
- Miron RJ Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland.
- Buser D Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland.
- Gruber R Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland.
- 2015-08-19
Published in:
- Journal of clinical periodontology. - 2015
Autografts, bone supernatant
TGF-β
antiseptic solution
bone
bone-conditioned medium
chlorhexidine
hydrogen peroxide
povidone-iodine
sodium hypochlorite
Animals
Anti-Infective Agents, Local
Bone and Bones
Cell Differentiation
Cell Survival
Cells, Cultured
Chlorhexidine
Culture Media, Conditioned
Disinfectants
Fibroblasts
Gingiva
Paracrine Communication
Sodium Hypochlorite
Swine
Transforming Growth Factor beta
English
AIM
Chemical decontamination increases the availability of bone grafts; however, it remains unclear whether antiseptic processing changes the biological activity of bone.
MATERIALS AND METHODS
Bone chips were incubated with four different antiseptic solutions including (1) povidone-iodine (0.5%), (2) chlorhexidine diguluconate (0.2%), (3) hydrogen peroxide (1%) and (4) sodium hypochlorite (0.25%). After 10 min. of incubation, changes in the capacity of the bone-conditioned medium (BCM) to modulate gene expression of gingival fibroblasts was investigated.
RESULTS
Conditioned medium obtained from freshly prepared bone chips increased the expression of TGF-β target genes interleukin 11 (IL11), proteoglycan4 (PRG4), NADPH oxidase 4 (NOX4), and decreased the expression of adrenomedullin (ADM), and pentraxin 3 (PTX3) in gingival fibroblasts. Incubation of bone chips with 0.2% chlorhexidine, followed by vigorously washing resulted in a BCM with even higher expression of IL11, PRG4 and NOX4. These findings were also detected with a decrease in cell viability and an activation of apoptosis signalling. Chlorhexidine alone, at low concentrations, increased IL11, PRG4 and NOX4 expression, independent of the TGF-β receptor I kinase activity. In contrast, 0.25% sodium hypochlorite almost entirely abolished the activity of BCM, whereas the other two antiseptic solutions, 1% hydrogen peroxide and 0.5% povidone-iodine, had relatively no impact respectively.
CONCLUSION
These in vitro findings demonstrate that incubation of bone chips with chlorhexidine differentially affects the activity of the respective BCM compared to the other antiseptic solutions. The data further suggest that the main effects are caused by chlorhexidine remaining in the BCM after repeated washing of the bone chips.
Chemical decontamination increases the availability of bone grafts; however, it remains unclear whether antiseptic processing changes the biological activity of bone.
MATERIALS AND METHODS
Bone chips were incubated with four different antiseptic solutions including (1) povidone-iodine (0.5%), (2) chlorhexidine diguluconate (0.2%), (3) hydrogen peroxide (1%) and (4) sodium hypochlorite (0.25%). After 10 min. of incubation, changes in the capacity of the bone-conditioned medium (BCM) to modulate gene expression of gingival fibroblasts was investigated.
RESULTS
Conditioned medium obtained from freshly prepared bone chips increased the expression of TGF-β target genes interleukin 11 (IL11), proteoglycan4 (PRG4), NADPH oxidase 4 (NOX4), and decreased the expression of adrenomedullin (ADM), and pentraxin 3 (PTX3) in gingival fibroblasts. Incubation of bone chips with 0.2% chlorhexidine, followed by vigorously washing resulted in a BCM with even higher expression of IL11, PRG4 and NOX4. These findings were also detected with a decrease in cell viability and an activation of apoptosis signalling. Chlorhexidine alone, at low concentrations, increased IL11, PRG4 and NOX4 expression, independent of the TGF-β receptor I kinase activity. In contrast, 0.25% sodium hypochlorite almost entirely abolished the activity of BCM, whereas the other two antiseptic solutions, 1% hydrogen peroxide and 0.5% povidone-iodine, had relatively no impact respectively.
CONCLUSION
These in vitro findings demonstrate that incubation of bone chips with chlorhexidine differentially affects the activity of the respective BCM compared to the other antiseptic solutions. The data further suggest that the main effects are caused by chlorhexidine remaining in the BCM after repeated washing of the bone chips.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1111/jcpe.12447
- PMID 26284317
- Persistent URL
- https://sonar.ch/global/documents/145
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