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Increase of aquaporin 9 expression in astrocytes participates in astrogliosis.
Journal article

Increase of aquaporin 9 expression in astrocytes participates in astrogliosis.

  • Hirt L Neurology Department, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland.
  • Price M Neurology Department, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland.
  • Mastour N Neurosurgery Research Group, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland.
  • Brunet JF Neurosurgery Research Group, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland.
  • Barrière G CNRS UMR 5287, INCIA, University of Bordeaux, Bordeaux, France.
  • Friscourt F CNRS UMR 5287, INCIA, University of Bordeaux, Bordeaux, France.
  • Badaut J CNRS UMR 5287, INCIA, University of Bordeaux, Bordeaux, France.
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  • 2017-04-19
Published in:
  • Journal of neuroscience research. - 2018
AQP9
astrogliosis
brain injury
mouse
preconditioning
thrombin
transient brain ischemia
water channel
Animals
Aquaporin 4
Aquaporins
Astrocytes
Cells, Cultured
Disease Models, Animal
Embryo, Mammalian
Gene Expression Regulation
Glial Fibrillary Acidic Protein
Gliosis
Green Fluorescent Proteins
Infarction, Middle Cerebral Artery
Mice
Mice, Inbred BALB C
Phosphorylation
RNA, Messenger
Serine
Transfection
English Here we assess the potential functional role of increased aquaporin 9 (APQ9) in astrocytes. Increased AQP9 expression was achieved in primary astrocyte cultures by transfection of a plasmid-containing green fluorescent protein fused to either wild-type or mutated human AQP9. Increased AQP9 expression and phosphorylation at Ser222 were associated with a significant change in astrocyte morphology, mainly with a higher number of processes. Similar phenotypic changes are observed in astrogliosis processes after injury. In parallel, we observed that in vivo, thrombin preconditioning before ischemic stroke induced an early increase in AQP9 expression in the male mouse brain. This increased AQP9 expression was also associated with astrocyte morphological changes, especially in the white matter tract. Astrocyte reactivity is debated as being either beneficial or deleterious. As thrombin preconditioning leads to a decrease in lesion size after stroke, our data suggest that the early increase in AQP9 concomitant with astrocyte reactivity leads to a beneficial effect. © 2017 Wiley Periodicals, Inc.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/149104
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