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Relative Centrifugal Force (RCF; G-Force) Affects the Distribution of TGF-β in PRF Membranes Produced Using Horizontal Centrifugation.

  • Kargarpour Z Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria.
  • Nasirzade J Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria.
  • Panahipour L Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria.
  • Miron RJ Department of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland.
  • Gruber R Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria.
  • 2020-10-20
Published in:
  • International journal of molecular sciences. - 2020
TGF-β
buffy coat
fibrin
gravitational force
platelet-poor plasma
platelet-rich fibrin
English Solid platelet-rich fibrin (PRF) is produced with centrifugation tubes designed to accelerate clotting. Thus, activated platelets may accumulate within the fibrin-rich extracellular matrix even before centrifugation is initiated. It can thus be assumed that platelets and their growth factors such as transforming growth factor-β (TGF-β) are trapped within PRF independent of their relative centrifugal force (RCF), the gravitation or g-force. To test this assumption, we prepared PRF membranes with tubes where clotting is activated by a silicone-coated interior. Tubes underwent 210 g, 650 g and 1500 g for 12 min in a horizontal centrifuge. The respective PRF membranes, either in total or separated into a platelet-poor plasma and buffy coat fraction, were subjected to repeated freeze-thawing to prepare lysates. Gingival fibroblasts were exposed to the PRF lysates to provoke the expression of TGF-β target genes. We show here that the expression of interleukin 11 (IL11) and NADPH oxidase 4 (NOX4), and Smad2/3 signaling were similarly activated by all lysates when normalized to the size of the PRF membranes. Notably, platelet-poor plasma had significantly less TGF-β activity than the buffy coat fraction at both high-speed protocols. In contrast to our original assumption, the TGF-β activity in PRF lysates produced using horizontal centrifugation follows a gradient with increasing concentration from the platelet-poor plasma towards the buffy coat layer.
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  • English
Open access status
gold
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https://sonar.ch/global/documents/152096
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