Journal article
Luciferases with Tunable Emission Wavelengths.
- Hiblot J Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
- Yu Q Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
- Sabbadini MDB Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
- Reymond L Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
- Xue L Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
- Schena A Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
- Sallin O Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
- Hill N Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
- Griss R Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
- Johnsson K Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
- 2017-09-24
Published in:
- Angewandte Chemie (International ed. in English). - 2017
bioluminescent imaging
luciferase
self-labeling protein
sensors
therapeutic drug monitoring
Biosensing Techniques
Fluorescent Dyes
Genes, Reporter
HeLa Cells
Humans
Luciferases
Luminescent Measurements
English
We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.1002/anie.201708277
- PMID 28941028
- Persistent URL
- https://sonar.ch/global/documents/166497
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