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Well-aligned chitosan-based ultrafine fibers committed teno-lineage differentiation of human induced pluripotent stem cells for Achilles tendon regeneration.
Journal article

Well-aligned chitosan-based ultrafine fibers committed teno-lineage differentiation of human induced pluripotent stem cells for Achilles tendon regeneration.

  • Zhang C Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, 310058, China.
  • Yuan H State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, China; College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.
  • Liu H Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, 310058, China.
  • Chen X Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, 310058, China.
  • Lu P Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, 310058, China.
  • Zhu T Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, 310058, China.
  • Yang L Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, 310058, China.
  • Yin Z Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, 310058, China.
  • Heng BC Department of Biosystems Science & Engineering (D-BSSE), ETH-Zurich, Mattenstrasse 26, Basel, 4058, Switzerland.
  • Zhang Y State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, China; College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China. Electronic address: yzzhang@dhu.edu.cn.
  • Ouyang H Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, 310058, China; Department of Sports Medicine, School of Medicine, Zhejiang University, Hangzhou, China; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. Electronic address: hwoy@zju.edu.cn.
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  • 2015-04-20
Published in:
  • Biomaterials. - 2015
Human induced pluripotent stem cells
Stepwise substrate change
Tendon differentiation
Tissue regeneration
Well-aligned chitosan ultrafine fibers
Achilles Tendon
Cell Differentiation
Cell Lineage
Chitosan
Humans
Induced Pluripotent Stem Cells
Regeneration
Surface Properties
Tissue Scaffolds
English Physical property of substrates such as stiffness and topography have been reported to induce mesenchymal stem cells differentiation into bone, muscle and neuron lineages. Human-induced pluripotent stem cells (hiPSCs) are a highly promising cell source for regenerative medicine. However, physical properties have not yet been reported to successfully induce pluripotent stem cells into specific lineages. This study aimed to develop a robust, stepwise topographic strategy to induce hiPSCs differentiate into teno-lineage. A novel spinning approach termed stable jet electrospinning (SJES), is utilized to fabricate continuous well-aligned ultrafine fibers (891 ± 71 nm), which mimic the native tendon's microstructure and mechanical properties. hiPSCs are first differentiated into MSCs on smooth plastic surface as confirmed by the differentiations into three mesenchymal lineages and expression of characteristic MSC surface markers through an EMT (Epithelial-Mesenchymal Transition) process. Subsequently, the hiPSC derived MSCs are seeded onto well-aligned fibers to differentiate into tenocyte-like cells through activating mechanic-signal pathway. The in situ tendon repair study further confirms that aligned fiber scaffold with hiPSC-MSCs had significant effect on improving the structural and mechanical properties of tendon injury repair. These findings indicate that the stepwise physical substrate change strategy can be adopted to induce hiPSCs differentiation for tendon tissue regeneration.
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  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/168138
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