Thymocytes can tolerize thymocytes by clonal deletion in vitro.
- Pircher H Department of Pathology, University of Zurich, Switzerland.
- Müller KP
- Kyewski BA
- Hengartner H
- 1992-09-01
Published in:
- International immunology. - 1992
Amino Acid Sequence
Animals
Apoptosis
CD4 Antigens
CD8 Antigens
Cells, Cultured
Immune Tolerance
Lymphocytic choriomeningitis virus
Mice
Mice, Transgenic
Molecular Sequence Data
Receptors, Antigen, T-Cell
T-Lymphocytes
Viral Proteins
English
Clonal deletion of thymocytes bearing TCR for self antigens is one major mechanism of T cell tolerance induction. Peptide antigen-induced deletion of thymocytes from alpha beta TCR transgenic mice has been studied using single cell suspension cultures. The results show that antigen-presenting immature CD4+CD8+ thymocytes can tolerize antigen-reactive immature thymocytes in vitro by programmed cell death (apoptosis) 6-8 h after antigen exposure. Antigen-induced apoptosis of immature thymocytes was inhibited by antibodies specific for the alpha beta TCR, CD3, CD8, and LFA-1 molecules. This implies that clonal elimination of self-reactive CD4+CD8+ thymocytes does not depend on specialized deleting cell types in the thymus and occurs whenever the TCR of immature thymocytes bind antigen fragments presented by MHC molecules.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1093/intimm/4.9.1065
- PMID 1390437
- Persistent URL
- https://sonar.ch/global/documents/185370
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