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Copper metallothionein in patients with hepatic copper overload.
Journal article

Copper metallothionein in patients with hepatic copper overload.

  • 1991-10-01
Published in:
  • European journal of clinical investigation. - 1991
Adolescent
Adult
Biliary Atresia
Child
Child, Preschool
Copper
Cytosol
Female
Hepatolenticular Degeneration
Humans
Liver
Liver Cirrhosis, Biliary
Male
Metallothionein
English We studied the Cu-MT present in the hepatic cytosol obtained from 7 patients suffering from conditions associated with hepatic Cu overload (Wilson's disease, biliary atresia, familial cholestatic cirrhosis). Since chromatographic methods appropriate for the isolation of Zn- and Cd-MT were unsuitable for Cu-MT, we developed an indirect procedure for the estimation and resolution of the latter. This procedure involved the preparation of apo-MT and its reconstitution to holo-MT with Zn or Cd. Three predominant isoforms of MT were present in all specimens. Our results indicate that at most 36 +/- 5% of the Cu present in the 10 kDa fraction of cytosol is bound to MT in the liver of patients with hepatic copper overload.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/185394
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