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Expression of tyrosinase in vegetative cultures of Neurospora crassa transformed with a metallothionein promoter/protyrosinase fusion gene.
Journal article

Expression of tyrosinase in vegetative cultures of Neurospora crassa transformed with a metallothionein promoter/protyrosinase fusion gene.

  • 1990-11-01
Published in:
  • Current genetics. - 1990
Cloning, Molecular
Copper
DNA, Fungal
Enzyme Induction
Enzyme Precursors
Gene Expression Regulation, Fungal
Genes, Fungal
Melanins
Metallothionein
Monophenol Monooxygenase
Neurospora crassa
Nucleic Acid Hybridization
Plasmids
Promoter Regions, Genetic
Recombinant Fusion Proteins
Transformation, Genetic
English Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is only expressed in situations of starvation or sexual differentiation. Complete integration of a 400 bp metallothionein promoter-fragment leads to constitutive expression of protyrosinase, whereas a 3.6 kb promoter-fragment conferred copper inducibility on the reporter gene in four transformants. A transformant with high constitutive tyrosinase levels was able to produce melanin on complete medium agar plates supplemented with 1 mg/ml L-tyrosine.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/185422
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