Journal article
Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis
- Furci, Leonardo ORCID P3 Centre for Plant and Soil Biology, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United Kingdom
- Jain, Ritushree P3 Centre for Plant and Soil Biology, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United Kingdom
- Stassen, Joost ORCID P3 Centre for Plant and Soil Biology, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United Kingdom
- Berkowitz, Oliver ORCID Department of Animal, Plant and Soil Science, ARC Centre of Excellence in Plant Energy Biology, La Trobe University, Melbourne, Australia
- Whelan, James Department of Animal, Plant and Soil Science, ARC Centre of Excellence in Plant Energy Biology, La Trobe University, Melbourne, Australia
- Roquis, David Institute for Advanced Study, Technical University of Munich, Garching, Germany
- Baillet, Victoire Institut de Biologie de l’Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), PSL Université Paris, Paris, France
- Colot, Vincent ORCID Institut de Biologie de l’Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), PSL Université Paris, Paris, France
- Johannes, Frank Institute for Advanced Study, Technical University of Munich, Garching, Germany
- Ton, Jurriaan ORCID P3 Centre for Plant and Soil Biology, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United Kingdom
- 2019-1-4
Published in:
- eLife. - eLife Sciences Publications, Ltd. - 2019, vol. 8
English
Variation in DNA methylation enables plants to inherit traits independently of changes to DNA sequence. Here, we have screened an Arabidopsis population of epigenetic recombinant inbred lines (epiRILs) for resistance against Hyaloperonospora arabidopsidis (Hpa). These lines share the same genetic background, but show variation in heritable patterns of DNA methylation. We identified four epigenetic quantitative trait loci (epiQTLs) that provide quantitative resistance without reducing plant growth or resistance to other (a)biotic stresses. Phenotypic characterisation and RNA-sequencing analysis revealed that Hpa-resistant epiRILs are primed to activate defence responses at the relatively early stages of infection. Collectively, our results show that hypomethylation at selected pericentromeric regions is sufficient to provide quantitative disease resistance, which is associated with genome-wide priming of defence-related genes. Based on comparisons of global gene expression and DNA methylation between the wild-type and resistant epiRILs, we discuss mechanisms by which the pericentromeric epiQTLs could regulate the defence-related transcriptome.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.7554/eLife.40655
- ISSN 2050-084X
- Persistent URL
- https://sonar.ch/global/documents/186352
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