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Semliki Forest virus and Sindbis virus vectors.
Journal article

Semliki Forest virus and Sindbis virus vectors.

  • 2008-04-23
Published in:
  • Current protocols in human genetics. - 2002
Animals
Cell Line
Cricetinae
Genetic Vectors
Genetics, Medical
Hippocampus
Humans
Neurons
Plasmids
Rats
Replicon
Semliki forest virus
Sindbis Virus
Tissue Culture Techniques
Transfection
Virus Assembly
English Semliki Forest virus (SFV) and Sindbis virus (SIN) are two, positive-strand RNA viruses of the alphavirus genus. Vectors for both have been developed to express high levels of foreign genes in vitro and in vivo. Basic Protocol 1 describes the preparation of packaged SFV and SIN replicons by co-electroporation of helper and vector RNA into baby hamster kidney (BHK)-21 cells. Basic Protocol 2 describes the activation of packaged SFV replicons with a-chymotrypsin. Basic Protocol 3 provides a method for the infection of hippocampal slices. Basic Protocol 4 is a technique for the infection of primary cultures of dispersed neurons with infectious SFV and SIN replicons. The Alternate Protocol describes a method for the cotransfection of in vitro-transcribed vector and helper RNA into BHK-21 cells. Support Protocol 1 describes determining the titers of infectious SFV and SIN replicon stocks, and Support Protocol 2 for metabolic labeling of infected cells.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/203942
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